Lecture 1 - Introduction of the microscope
Lecture 2 - Image formation by glass lenses and its role in wavelength
Lecture 3 - Different components of electron microscope (EM)
Lecture 4 - Electron specimen interaction
Lecture 5 - Elements of TEM: electron gun - I
Lecture 6 - Elements of TEM: electron gun - II
Lecture 7 - Comparison between the various types of electron gun
Lecture 8 - Elements of TEM: electromagnetic lens (EM) - I
Lecture 9 - Trajectory of electrons in EM lens
Lecture 10 - Elements of TEM: electromagnetic lens (EM) - II
Lecture 11 - Image Formation by EM Lenses: Understanding Ray Diagrams
Lecture 12 - Aberrations and Focusing in Cryo-EM imaging
Lecture 13 - Understanding Chromatic, Spherical Aberrations, and Astigmatism in Cryo-EM
Lecture 14 - Aberrations and Practical Imaging in Cryo-EM
Lecture 15 - Electron Dose Control and Beam Optimization in Cryo-EM
Lecture 16 - Introduction to TEM Holders and Grids in Cryo-EM
Lecture 17 - Overfocused and Underfocused Images
Lecture 18 - Elements of TEM: Pumps in EM System
Lecture 19 - Elements of TEM: Detectors in EM System
Lecture 20 - Imaging and Effect of Thermal Drift
Lecture 21 - Wave and its properties
Lecture 22 - Addition/superposition of waves - I
Lecture 23 - Addition/superposition of waves - II
Lecture 24 - Decomposition of complex waves
Lecture 25 - Wave and their transformation
Lecture 26 - Complex 2D Images and Its Diffraction Pattern
Lecture 27 - Sample Preparation and Determination for TEM
Lecture 28 - Biological Sample Purification - I
Lecture 29 - Biological Sample Purification - II
Lecture 30 - Different Sample Preparation Techniques for TEM Imaging and Different Types of Grids used for Imaging
Lecture 31 - TEM Grids: Types, Handling and Surface Coatings
Lecture 32 - Carbon Coaters, Glow Discharge and Grid Surface Treatment
Lecture 33 - Negative Staining in TEM: Stains, Grid Preparation and Protocols
Lecture 34 - Carbon Sandwich and Metal Shadowing Techniques in TEM
Lecture 35 - Staining Quality in TEM: Thick, Thin and Positive Staining
Lecture 36 - Negative Staining - Imaging Quality and Artifacts
Lecture 37 - Negative Staining - Particle Distribution and Contrast
Lecture 38 - Applications of Negative Staining in Structural Biology
Lecture 39 - Negative Staining for Complexes and Interactions
Lecture 40 - Ultramicrotomy and Sectioning for Biological Molecules
Lecture 41 - Cryo-EM Grid Preparation and Vitrification
Lecture 42 - Grid Types and Sample Distribution in Cryo-EM
Lecture 43 - Blotting Parameters and Ice Conditions
Lecture 44 - Motion Correction and Defocus in Cryo-EM
Lecture 45 - Microscope Alignment and Imaging Limitations
Lecture 46 - CTF Estimation and Defocus in Cryo-EM
Lecture 47 - Phase Flip and Motion Correction
Lecture 48 - High Pressure and Rapid Freezing Methods
Lecture 49 - Cryo-Ultra Microtomy and FIB-SEM
Lecture 50 - Graphene Oxide Grids in Cryo-EM
Lecture 51 - Motion Correction and 2D Classification Basics
Lecture 52 - Concepts and Methods in 2D/3D Classification
Lecture 53 - 3D Reconstruction and Resolution Assessment
Lecture 54 - Practical: TEM Setup and Negative Staining
Lecture 55 - Practical: Cryo-EM Plunge Freezing
Lecture 56 - Practical: Cryo-EM Grid Clipping
Lecture 57 - Practical: Grid Loading and Imaging Software
